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. 2015 Jun;185(6):1624–1637. doi: 10.1016/j.ajpath.2015.02.018

Figure 2.

Figure 2

Intestinal epithelial necrotic cell lysate (NCL)–induced IL-6 and IL-8 production by human intestinal fibroblasts (HIFs) is mediated by IL-1 receptor (IL-1R) and IL-1α but not IL-1β. A: HIFs were incubated with 2.5 μg/mL neutralizing antibody to type I IL-1R (IL-1RI) for 1 hour, followed by HT29 NCLs (diluted 1:20) for 24 hours. B and C: HIFs were incubated for 24 hours with HT29 NCLs (diluted 1:20) preincubated for 1 hour with 0.1 μg/mL neutralizing anti-human IL-1α antibody (B) or with 2.5 μg/mL neutralizing anti-human IL-1β antibody (C). IL-6 and IL-8 levels in the supernatants were measured by enzyme-linked immunosorbent assay. n = 3 (A–C). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.