Table 3.
Additional Markers Investigated by IHC or IF in Primary Cultures (>20 Passages) of Mucin- or Mixed-IHCCA
| Markers (technique) | Mucin-IHCCA | Mixed-IHCCA |
|---|---|---|
| E-cadherin (IHC-IF) | 0% | 0% |
| CD31 (IHC-IF) | 0% | 0% |
| GFAP (IHC) | 0% | 0% |
| CD163 (IHC) | 0% | 0% |
| SDF1 (IF) | 0% | 0% |
| FAP (IF) | 0% | 0% |
| Periostin (IF) | 18 ± 3.0% | 14 ± 3.5% |
| α-SMA (IHC-IF) | 20 ± 3.6% | 15 ± 4.3% |
| S100A4 (IHC-IF) | 100% | 100% |
| SNAIL (IHC-IF) | 88 ± 3.2% | 86 ± 3.5% |
| TWIST (IHC-IF) | 86 ± 1.5% | 85 ± 2.5% |
| NCAM (IF) | 98 ± 2.0% | 96 ± 3.0% |
| CK19 (IF) | 13 ± 3.0% | 12 ± 2.7% |
| Vimentin (IHC-IF) | 100% | 100% |
| Nestin (IF) | 100% | 100% |
Data indicate percentage of cells positive and are expressed as means ± SD.
α-SMA, α-smooth muscle actin; CK, cytokeratins; FAP, fibroblast-activation protein; GFAP, glial fibrillary acidic protein; IF, immunofluorescence; IHC, immunohistochemistry; IHCCA, intrahepatic cholangiocarcinoma; NCMA, neural cell adhesion molecule; SDF1, stromal-derived factor.