Fig. 1.

OR47b- and OR88a-expressing OSNs detect ML, MM, and MP. (A) Average SSR responses from all OSNs housed in trichoid sensilla after stimulation with 42 cuticular compounds (10⁻¹ dilution) (n = 5). (B) Representative SSR traces from measurements of WT at4 OSNs stimulated with ML, MM, and MP (10⁻¹ dilution). (C) Representative GC-SSR measurements from at4 OSNs stimulated with GC-fractionated fly body wash extracts (n = 4). (D) Heat map of the average SSR responses from all OSN classes stimulated with ML, MM, and MP (10⁻¹ dilution) (n = 3). Asterisks denote the total activity of an OSN when spike sorting failed. (E) Dose–response curves from at4A and at4C OSNs to ML, MM, and MP (n = 5). (F) Average SSR responses from ∆ab3A: Or47b, ∆ab3A: Or65a, and ∆ab3A: Or88a to ML, MM, and MP (10⁻¹ dilution) (n = 5). (G) Representative SSR traces from Δab3A: Or47b and Δab3A: Or88a stimulated with ML and MM (10⁻¹ dilution). (H) Average SSR responses from at4A and at4C OSNs of Or47b[3] mutant flies stimulated with ML, MM, and MP (10⁻¹ dilution) (n = 5). (I) Representative SSR traces from at4 OSNs of Or47b[3] mutant flies stimulated with ML, MM, and MP (10⁻¹ dilution). (J) Average SSR responses from at4A and at4C OSNs of Or88a^−/− mutant flies stimulated with ML, MM, and MP (10⁻¹ dilution) (n = 5). (K) Representative SSR traces from at4 OSNs of Or88a^−/− mutant flies stimulated with ML, MM, and MP (10⁻¹ dilution).