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Effect of PACAP38 on GluA1 phosphorylation. (A) Hippocampal cultures (DIV 14) were stimulated with different concentrations (nM) of PACAP38 for 10 min. Stimulation was followed by cell lysis and Western blot analysis. (B) GluA1 was immunoprecipitated from whole brain lysate prepared from WT and “penta” knock-in mice. Input and GluA1 IP samples were visualized by Western blot.
