Fig. 4.

Identification of kinases or phosphatases responsible for PACAP38-dependent GluA1 phosphorylation changes. Hippocampal neurons (DIV 14) were preincubated with 10 µM H89 for 10 min (A), with 1 μM Go6983 for 10 min (B), with 2 µM okadaic acid (OA) for 10 min (C), or with 2 µM cyclosporine A (CsA) for 15 min (D), and then stimulated with PACAP38 (1 nM) for 10 min. Cells were lysed, GluA1 immunoprecipitated, and samples visualized by Western blot. (E–H) Quantification of GluA1 T840 or S845 phosphorylation normalized to GluA1. Error bars indicate ±SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA, Bonferroni posttest. n ≥ 6.