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NMDA receptor involvement in GluA1 phosphorylation changes. (A) Hippocampal neurons (DIV 14) were preincubated with D-APV (50 µM) for 45 min and then stimulated with PACAP38 (1 nM) for 10 min. Cells were lysed, GluA1 was immunoprecipitated, and samples were examined by Western blot. (B) Quantification of GluA1 T840 or GluA1 S845 phosphorylation normalized to GluA1. Error bars indicate ±SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA, Bonferroni posttest. n ≥ 6.
