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. 2015 May 6;112(21):6694–6699. doi: 10.1073/pnas.1421804112

Fig. 3.

Fig. 3.

Structure of the LANA spiral. (A) Top view of the spiral crystal structure of wild-type LANA(1013–1149) shown in cartoon representation (Left) and in an electrostatic surface potential representation (Right). (B) Side view of a full helical repeat of 24 dimers showing the electrostatic surface potential on the outside (Left) and inside (Right) of the spiral. (C and D) Negative-stain EM of a specific DNA-binding-deficient LANA(1013–1162) multiple-point mutant bound to 207 bp/70 nm nonspecific DNA. Kinks in the regular complexes are marked in D. (E) A LANA DBD mutant as in C and D additionally carrying the A1121E mutation loosely bound to 207 bp/70 nm DNA. (F) A LANA DBD mutant as in C and D additionally carrying the mutations K1055E, K1109E, and K1138E in the presence of 207 bp/70 nm DNA. (G) The same mutant as in C and D in the absence of DNA.