Fig. 2.

False-color heat map and hierarchical clustering of small molecules detected in single D11, V11, and V21 blastomeres of 16-cell Xenopus embryos. Metabolite abundances are normalized relative to the mean and shown in false color for the 40 statistically most significant features (P < 0.05). Each blastomere has a unique identifier (bottom axis) with solid lines connecting the technical replicates for four of the n = 5 biological replicates that were measured for each cell type. The data reveal that D11, V11, and V21 cells have distinct small-molecular activities during early embryogenesis. Abbreviations of small molecules are given in SI Appendix, Table S2.