. 2015 Apr 27;10:12. doi: 10.1186/s13064-015-0039-5

Table 1.

Generation of chimeras by morula aggregation

Aggregation type	Number of morula aggregations attempted	Number of embryos
Aggregation type	Number of morula aggregations attempted	Implanted ^a	Recovered	Evaluated for chimerism	Identified as chimeric ^b
1↔1 aggregations
Vsx2 ^wt↔Yfp ⁺	58	44	8^c	5	3
Vsx2 ^orJ↔Yfp ⁺	77	60	19^d	13	11
2↔1 aggregations
Vsx2 ^wt↔Yfp ⁺	120	131^e	23^f	21	11
Vsx2 ^orJ↔Yfp ⁺	128	128^g	13^h	12	10

^aAll successfully aggregated and partially aggregated (successful aggregation of 2 of the 3 morulae during 2↔1 morula aggregations) embryos were implanted into pseudopregnant females; ^bchimeras identified by evaluation of Yfp ⁺ contribution in eye or limb sections; ^c3 embryos exhibited light or absent eye pigmentation and were excluded from further analysis; ^d5 embryos exhibited light or absent eye pigmentation and were excluded from further analysis; ^eincludes 102 successfully aggregated embryos, 18 partially aggregated embryos, and 11 nonaggregated embryos implanted as fillers; ^f1 embryo was grossly underdeveloped and 1 embryo lacked eye pigmentation. Both embryos were excluded from further analysis; ^gincludes 112 successfully aggregated embryos and 16 partially aggregated embryos; ^h1 embryo exhibited abnormal gross morphology and was excluded from further analysis.