Table 1.
Primer set | Primer sequences | Amplicon size (bp) | Sequence source |
---|---|---|---|
rhA1AT | 5′-GCAATGCTACCGCAATTTTCTT-3′ | 72 | Primer Express Software 3.0 |
5′-CATGGGTCAGCTCGTTTTCC-3′ | (Applied Biosystems) | ||
dhfr | 5′-ACCAGGCCACCTCAGACT-3′ | 120 | Ng et al., 2007 [31] |
5′-GAGAGGACGCCTGGGTATT-3′ | |||
β-actin | 5′-AGCTGAGAGGGAAATTGTGCG-3′ | 163 | Fox et al., 2005 [52] |
5′-GCAACGGAACCGCTCATT-3′ |
rhA1AT primer set was verified by sequencing the product obtained from PCR with the first-strand cDNA from a stably transfected CHO cell line as template.