Fig. 2.

Cultures of cells derived from deciduous teeth. (A) Tooth fragments are taken into culture in a 12-well plate under standard fibroblast cell culture conditions. (B) Fibroblast-like cells were observed in the culture plate after approximately 2 weeks (left) and expanded ~1 week (right) at ×4 magnification. Scale bar 200 μm. (C) Immunofluorescence imaging of fibroblast-like cells from healthy donors (n = 3) shows 25–51 % ciliation after 24-h serum starvation. Cells from one donor are shown here. Nucleus (DAPI, blue); ARL13B and INPP5E (red); Ac. Tubulin (green). Scale bar 5 or 10 μm. (D). Imaging of fibroblast-like cells from a healthy donor showing cilia and centrosome. Nucleus (DAPI, blue); Ac. Tubulin (green); pericentrin (PCNT; red). Scale bar 10 μm