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. 2015 Jun 2;16(1):424. doi: 10.1186/s12864-015-1585-2

Figure 1.

Figure 1

Schematic representation of the specificity switching of the Type IIG MTase HpyUM032XIII. (A) The S1 and S2 subunits are separated by a homopolymeric tract of 12 G residues at the location shown by ▼, which appears to create a natural frameshift. (B) Reducing the tract length from 12 to 11 corrects the frameshift at ▼, thereby fusing the S1 and S2 subunits. This ‘corrected’ sequence is denoted by ‘mut’, to create fusion of S1 and S2 subunits. (C–D) Expression of individual S subunits with the RM gene shows that the S1 subunit is active in the absence of S2.