Figure 3.

Retained roles of FVIII and FIX in the formation of platelet-fibrin thrombi and procoagulant activity without tissue factor. Blood from mice deficient in FVIII or FIX, or from corresponding wild-type mice was perfused over collagen under coagulant conditions. Where indicated, blood samples were pre-incubated with tissue factor (TF, 10 pM) and OG488-fibrinogen. Thrombi were then stained with FITC-annexin A5 as in Figure 1. (A) Representative microscopic images after 4 min of flow of F8^−/− or F9^−/− blood in the absence of TF (bars, 50 μm). (B) Effect of TF on parameters of thrombus formation in F8^+/+ and F8^−/− mice. (C) Effect of TF on parameters of thrombus formation in F9^+/+ and F9^−/− mice. Mean ± SEM; n = 3–7; *P<0.05 vs. corresponding wild-type.