Figure 7.
Maintained defective formation of platelet-fibrin thrombi with hemophilic blood at high tissue factor concentrations. Blood from normal control donors or a patient with hemophilia A (HA) or hemophilia B (HB) was perfused over collagen at a shear rate of 1000 s−1 for up to 20 min. Blood samples were pre-incubated with DiOC6 to label platelets, AF647-fibrinogen and different concentrations of tissue factor (TF, 0–100 pM), as indicated. (A, B) Effect of TF concentration on surface area covered by (A) platelets and (B) fibrin(ogen) with normal blood samples. (C) Effect of TF concentration on time to first fibrin formation. (D) Prolonged time to first fibrin formation with blood from a HA or HB patient. Mean ± SEM; n = 4–6; *P<0.05 compared to normal controls.