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. 2015 Jun;100(6):757–767. doi: 10.3324/haematol.2014.118497

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Figure 3. — CD9 controls Akt/MAPK balance during PMF dysmegakaryopoiesis. Analysis by cytometry, microscopy and western blot of (A) phospho-Akt (Thr308 and Ser473), - GSK3β (Ser9) levels (MFI), or (B) phospho-p38 (Thr180 and Tyr182) and phospho-JNK levels (MFI) in megakaryocytes (Mk) derived from PMF circulating CD34⁺ cells (D6 of culture in the presence of Tpo) at 2, 24, and 48 h after anti-CD9 monoclonal antibody (mAb) (clone-SYB; 10 μg/mL) treatment. (A and B) Data were normalized either to IgG control values or to total protein level. (B) QRT-PCR analysis of AP1 transcript expression level in Mk derived from PMF circulating CD34⁺ cells, treated or not with anti-CD9 mAb (clone-SYB; 10 μg/mL) and normalized to actin level.