Table 1.

Amino acid composition and flexibility analysis of enolases from different microorganisms.

Organism	Growth temp, °C	pI	Amino acid composition, %							Flexibility analysis
			Amino acids				Residues		AIf	H bonds	Rc	#Rc	df
			Hydrophobica	Chargedb	Polarc	Gly	Negd	Pose
P. furiosus	100	4.98	48.8	27.4	14.7	9.1	64	45	97.74	295	1657	155	622
T. maritima	80	4.93	47.2	28	16.6	8.2	66	48	98.01	290	1575	526	656
T. aquaticus	70	5.01	47.5	27.7	13.7	11.1	64	47	98.27	296	1590	496	590
C. aurantiacus	55	4.99	48.3	25.1	17.4	9.2	58	42	99.25	354	915	3405	525
E. coli	37	5.32	45.6	26.4	17.1	10.9	59	48	89.12	344	1468	908	535
C. difficile	37	4.58	46.7	26	16.3	10.9	68	41	96.42	343	1389	1608	496
P. falciparum	37	6.21	45.8	25.1	21.7	7.4	55	53	97.35	361	905	3980	502
C. albicans	37	5.54	46.5	25.7	18.9	8.9	57	49	92.09	392	911	3620	455
S. cerevisiae	30	6.17	46	27.1	18.4	8.5	56	51	90.69	405	852	3826	406
T. brucei	27	5.93	43.8	26.6	20	9.6	56	51	84.41	399	589	4647	386

^aTotal number of hydrophobic amino acids: alanine, leucine, isoleucine, valine, proline, phenylalanine, tyrosine, tryptophan, and methionine.

^bTotal number of charged amino acids: asparagine, glutamate, arginine, and lysine.

^cTotal number of polar amino acids: asparagine, glutamine, serine, threonine, and cysteine.

^dTotal number of negatively charged amino acids: asparagine and glutamate.

^eTotal number of positively charged amino acids: arginine and lysine.

^fAI – The aliphatic index of proteins is defined as the relative volume occupied by aliphatic side chains (alanine, valine, isoleucine, and leucine)

pI, principle isoelectric point; neg, negative; pos, positive; AI, aliphatic index; H bonds, hydrogen bonds; Rc, rigid clusters; #Rc, total number of sites in rigid cluster; df, total independent degrees of freedom.

Bold font indicates that the data presented in the tables are from this work.