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The purification of EnoCa.
| Stages of purification | Total protein, mg | Activity |
Degree of purification | ||
|---|---|---|---|---|---|
| Specifica, U/mg | Total, units | Yield, % | |||
| Crude extract | 570.1 ± 13.5 | 0.76 ± 0.03 | 433 ± 15 | 100 | 1 |
| Chromatography on Q-sepharose | 250.3 ± 9.8 | 1.28 ± 0.05 | 320 ± 12 | 74 ± 2 | 2 ± 0 |
| Gel filtration on sephacryl S-300 | 20.8 ± 0.8 | 12.4 ± 0.3 | 258 ± 11 | 59 ± 2 | 16 ± 1 |
| Chromatography on octyl-sepharose | 1.1 ± 0.1 | 147 ± 6 | 162 ± 7 | 37 ± 1 | 213 ± 2 |
a1 unit of enolase activity (μmol PEP min−1) was measured at 25°C.
