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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Clin Immunol. 2010 Nov 2;31(2):228–239. doi: 10.1007/s10875-010-9476-4

Fig. 3.

Fig. 3

Functional iNKT cells are present in pig lungs. Pig lung MNCs and PBMCs (control) were cultured in the presence of α-GalCer (1,000 ng/ml) or vehicle for 12 days. a Immunostained using fluorochrome conjugated anti-pig CD3ε and mouse empty or PBS-57 loaded CD1d tetramers and analyzed by flow cytometry. b Culture supernatants harvested on post-6 and 12 day cultures were analyzed for IFNγ and IL-4 using pig cytokine-specific ELISA. Each bar represents the average cytokines OD from three pigs±SEM and * denote statistically significant difference (P<0.05) in the amount of cytokines secreted by cells cultured in the presence of vehicle vs α-GalCer. The data shown are representative of six pigs in three independent experiments