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. 2012 Apr 15;125(8):1896–1909. doi: 10.1242/jcs.090571

Fig. 5.

Fig. 5.

p115/1-766 inhibits VSV-G traffic. (A–C) HeLa cells transfected with GFP-p115/1-766 for 24 hours were transfected with ts045-VSV-G at 42°C, cultured for additional 12 hours and either analyzed directly (A) or shifted to 36°C for 1 hour (B) or 2 hours (C) and processed for immunofluorescence to detect VSV-G and p115/1-766. VSV-G is largely retained within punctate fragments after 1 hour (B) and 2 hours at 36°C (C). Scale bars: 10 µm. (D–H) HeLa cells silenced with anti-p115 siRNA for 3 days were co-transfected at 42°C with ts045VSV-G and either Myc-p115/1-959 (D,F) or Myc-p115/1-766 (E,G–H), cultured for additional 12 hours, shifted to 36°C for indicated times and analyzed by immunofluorescence. In p115-depleted cells replaced with full-length p115, VSV-G traffics to the Golgi and the plasma membrane (F). In p115-depleted cells replaced with p115/1-766, VSV-G is retained within punctate fragments after 2 hours (G) and after 12 hours (H). Scale bars: 10 µm.