Figure 4.

BPIFA1 protein levels are modulated after MHV-68 infection. Lungs were harvested from wood mice that were either mock- or MHV-68-infected at either day 7 or day 14 p.i. as indicated. BPIFA1 was detected by IH analysis using antibodies specific for BPIFA1, visualized with DAB, and counterstained with hematoxylin. The intensity of staining (a) and percentage area of epithelium stained (b) in the trachea, bronchi, and bronchioles were assessed by image analysis. Data are for three mice per group presented as mean±s.e.m. (n=3). Significant differences from mock-infected mouse values are shown by an asterisk.