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. 2015 Jun 1;5:10581. doi: 10.1038/srep10581

Figure 6. Laser mediated damage is via heat and ROS in vivo.

Figure 6

(a) Images of the dorsal portion of 5-week-old C57BL/6NCr male mice treated with laser (55 °C) or following skin cooling for 30 s. Quantitation of damaged area (b) and erythema score (c) are shown (n = 5). (d and e) Representative image of Reactive Oxygen Species (ROS) induction as measured with ROSstar probe using IVIS in vivo imaging is shown (Radiation is used as a positive control). Treatment sites were pretreated with NAC or NAA and ROS level was quantitated after laser treatment (f). Dorsal area was photographed (g) and damaged area (h) and erythema (i) are shown (n = 5). (J-m) Laser treated mice skin tissues from these experiments were assessed with immunostaining for ATF-4 and HSP70 expression and their quantitation was performed with ImageJ (n = 5). Significance was assessed with paired Student’s t-test and denoted as P < 0.05 (*), <0.001 (**), <0.0001 (***), <0.00001(****) and not significant (n.s.).