Figure 4. Impact of Mycobacterium smegmatis respiratory chain mutations on the susceptibility for clofazimine.

Strains of M. smegmatis were treated with the indicated amounts of clofazimine for 72 hours and CFU/mL were counted on agar plates after three (wild-type, ΔcydA :: kan) or four days (ΔqcrCAB :: hyg) of incubation. Black bars, wild-type; grey bars: ΔqcrCAB :: hyg; white bars: ΔcydA :: kan. Error bars represent means of at least two independent experiments with standard error of the mean (SEM).