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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Thorax. 2008 Dec 3;64(4):313–320. doi: 10.1136/thx.2008.096222

Figure 4.

Figure 4

(A) Flow cytometry density plot showing the gating of sputum phagocytic cell populations based on light scatter properties. The right panel shows histograms of fluorescence intensity of gated macrophage populations (combined mature and immature macrophages) from (B) a normal subject and (C) a subject with asthma. Cells in the M1 region did not engulf particles while cells which engulfed particles had a higher mean fluorescence intensity (MFI) and shifted into the M2 region. Subjects with asthma were more efficient in phagocytosis of opsonised zymosan-A bioparticles (higher MFI) than healthy subjects. FSC, forward scatter; SSC, side scatter; PMN, polymorphonuclear leucocyte (neutrophil); MAC, macrophage.