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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Biol Chem. 2003 Jul 16;278(39):38084–38093. doi: 10.1074/jbc.M307028200

Fig. 3. Activation of vitamin D3 by CYP2R1.

Fig. 3

The indicated vitamin D receptor-reporter systems were introduced into HEK 293 cells grown in 60-mm dishes together with the expression plasmids designated at the bottom of the figure. Relative luciferase enzyme activity was determined 16–20 h later as indicated under “Experimental Procedures,” and the means ± S.E. for experiments carried out in triplicate were plotted as histograms. The amounts of vitamin D3 added to the medium were 0.5 μM (A and B) and 1 μM (C).