Figure 7.

Parasympathetic nucleus of the vagus; cat, secondary passage Fel^CWD, case No. 1. Finely granular to clumped misfolded prion protein (PrP^D) deposits (red) are seen dispersed through the tissue, and dual immunofluorescence with the anti–lysosome antibody Lamp1 (green) reveals extensive accumulation of PrP^D with lysosomes (yellow). The yellow color represents the overlapping of red and green immunofluorescent staining, and nuclei are stained with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI; blue). Inset: higher magnification further illustrates the association between PrP^D and Lamp1-positive lysosomes.