TABLE 3. Primer Sequences for Reverse Transcription-Polymerase Chain Reaction Analysis.
| Gene | Primer | Sequences | TA (°C) | Cycles |
|---|---|---|---|---|
| RUNX-2 | Forward | ACGACAACCGCACCATGGT | 59 | 30 |
| Reverse | CTGTAATCTGACTCTGTCCT | |||
| ALP | Forward | TGGAGCTTCAGA AGCTCAACACCA | 59 | 28 |
| Reverse | ATCTCGTTGTCTGAGTACCAGTCC | |||
| OC | Forward | ATGAGAGCCCTCACACTCCTC | 59 | 30 |
| Reverse | GCCGTAGAAGCGCCGATAGGC | |||
| PPAR-2 | Forward | CGAGGGCGATCTTGACAGGAA | 55 | 30 |
| Reverse | CAGGGGGGTGATGTGTTTGAAC | |||
| APP | Forward | CTGTCCAAGTCCAACAGCAA | 55 | 30 |
| Reverse | ACGTTGGCAGCTTTACGTCT | |||
| LPL | Forward | TCCGCGTGATTGCAGAGAGAG | 55 | 30 |
| Reverse | TGCTGCTTCTTTTGGCTCTGACT | |||
| AGG | Forward | TGAGGAGGGCTGGAACAAGTACC | 57 | 28 |
| Reverse | GGAGGTGGTAATTGCAGGGAACA | |||
| COL II | Forward | TTTCCCAGGTCAAGATGGTC | 57 | 30 |
| Reverse | TCACCTGGTTTTCCACCTTC | |||
| SOX-9 | Forward | TGGCCGAGATGATCCTAAAAATAA | 57 | 30 |
| Reverse | GCGCTTGGATAGGTCATGTTTGT | |||
| β-actin | Forward | GTGGGGCGCCCCAGGCACCA | 55 | 45 |
| Reverse | CTTCCTTAATGTCACGCACGATTTC |