TABLE 4. Primer Sequences for Reverse Transcription-Polymerase Chain Reaction Analysis.

Gene	Primer	Sequences (5′–3′)
ACAN	Forward	TGAGGAGGGCTGGAACAAGTACC
	Reverse	GGAGGTGGTAATTGCAGGGAACA
COL2A1	Forward	TTTCCCAGGTCAAGATGGTC
	Reverse	TCACCTGGTTTTCCACCTTC
SOX-9	Forward	TGGCCGAGATGATCCTAAAAATAA
	Reverse	GCGCTTGGATAGGTCATGTTTGT
KRT19	Forward	AACGGCGAGCTAGAGGTGA
	Reverse	GGATGGTCGTGTAGTAGTGGC
CA12	Forward	CGTGCTCCTGCTGGTGATCT
	Reverse	AGTCCACTTGGAACCGTTCACT
FOXF1	Forward	TCCCTGGAGCAGCCGTATC
	Reverse	TGGGCGACTGCGAGTGATA
HIF-1α	Forward	CCCTCACCCAACGAAAAATTAC
	Reverse	GGGACTATTAGGTGAAC
SOX-2	Forward	CCCCCCTGTGGTTACCTCTTC
	Reverse	GGCCGCTCTGGTAGTGCTG
NANOG	Forward	ACCCCGTTTCACTGTGTTAGC
	Reverse	GACGGCAGCCAAGGTTATTAAA
OCT4	Forward	GGCAAGCGATCAAGCAGCGAC
	Reverse	GGGAAAGGGACCGAGGAGTAC
β-actin	Forward	GTGGGGCGCCCCAGGCACCA
	Reverse	CTTCCTTAATGTCACGCACGATTTC

ACAN indicates aggrecan; COL2A1, collagen II α 1; KRT19, cytokeratin-19; CA12, carbonic anhydrase XII; FoxF1, forkhead box F1.