Figure 2c:
Images derived from CEST MR imaging of G47Δ–empty virus and G47Δ-LRP–infected cell lysates. (a) Representative MTRasym map for phantoms that contained lysates of D74/HveC cells infected with either G47Δ–empty virus (upper half of the image) or G47Δ-LRP (lower half of the image). (b) Graph shows quantification of the MTRasym induced by G47Δ-LRP in these cells. Significantly (P = .01) higher MTRasym was observed in D74/HveC cell lysates infected with G47Δ-LRP (1.52% ± 0.06) compared with G47Δ–empty virus (1.0% ± 0.02). (c) Photomicrographs (lacZ staining for β-galactosidase activity; original magnification, ×10) show that staining for viral β-galactosidase activity in cells infected with G47Δ-LRP or G47Δ–empty virus indicate equal spread for the two viruses (approximately 20% of cells were infected 18 hours after infection).