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. 2015 May 5;226(5):478–488. doi: 10.1111/joa.12296

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Fig 2 — Col VI-immunohistochemistry of the femoral diaphysis. (A) At 1 day old, faint fibrous immunoreactivity (arrowheads) is detected in the ECM in the vascular cavity (VC) of the cortical bone (CB). (A′) A high-magnification view of the boxed area. (B) At 2 weeks, the immunoreactivity (arrows) is more intense in the ECM in the osteonal cavities (OC) of incomplete (ICPO) and complete primary osteons (CPO). (C) A high-magnification view of the periosteal cortical region of (B). In the ICPOs, bone trabeculae (dotted line) extend to enclose the immunoreactive areas (arrows). (D) At 7 weeks old, immunoreactive ECM (arrows) is decreased in the CPOs as the OCs become smaller from the outer region toward the endosteal region. (E) At 3 months old, no immunoreactivity is observed. PeO, periosteum; BM, bone marrow; asterisk, blood vessel. Scale bars: 50 μm.