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. 2015 May 5;226(5):478–488. doi: 10.1111/joa.12296

Fig 4.

Fig 4

Runx2-immunohistochemistry of the femoral diaphysis. (A) and (B) At 2 (A) and 7 (B) weeks old, the immunoreactivity is detected in the cuboidal osteoblasts (arrowheads) and spindle cells (arrows) in the osteonal cavity (OC) of the incomplete (ICPO) and complete primary osteons (CPO). (C) At 3 months old, no immunoreactive cells are detected. (D–F) Double-labeling immunohistochemistry for Col VI (green) and Runx2 (red) shows Runx2-immunoreactive spindle cells (arrow) and cuboidal osteoblasts (arrowhead) in the Col VI-immunoreactive ECM in the primary osteon. CB, cortical bone; asterisk, blood vessel. Scale bars: 20 μm. (G) Runx2 labeling index in the osteonal cavity and osteogenic layer at 2, 4, 7 weeks and 3 months. Data represent mean ± SD. Significant differences were analyzed by Student's t-test (osteonal cavity vs. osteogenic layer; N.S. not significant, *P < 0.05, **P < 0.01) or one-way anova and Tukey post hoc test (between the 2, 4, 7 weeks and 3 months; +,#P < 0.05).