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. 2015 May 5;226(5):478–488. doi: 10.1111/joa.12296

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Fig 6 — NG2-immunohistochemistry of the femoral diaphysis. (A) At 2 weeks, NG2 immunoreactivity is found in the cuboidal osteoblasts (arrowheads) and spindle cells (arrows) in the osteonal cavity (OC) of the incomplete (ICPO) and complete primary osteons (CPO). (B) At 7 weeks, the immunoreactive spindle cells (arrows) and osteoblasts (arrowheads) are detected mainly in the ICPOs. (C) At 3 months, a few or no NG2-immunoreactive spindle cells (arrows) are detected in the CPO. (D–F) Double-labeling immunohistochemistry for NG2 (green) and Runx2 (red) shows NG2-immunoreactive spindle cells (arrow) and cuboidal osteoblasts (arrowhead) express Runx2. (G–I) Double-labeling immunohistochemistry for NG2 (green) and Col VI (red) shows NG2-immunoreactive spindle cells (arrow) and cuboidal osteoblasts (arrowhead) in the Col VI-immunoreactive ECM. (J) A high-magnification view of the boxed area. Asterisk, blood vessel. (A–I) Scale bars: 20 μm. (J) Scale bar: 10 μm.