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Bioinformatic pipeline for metagenomics. Reads were prefiltered and removed if they were low quality/complexity (∼7%), ribosomal RNA (∼69%), or mitochondrial RNA (∼6%). The remaining reads were assigned as human (∼17%) including HERV (∼0.006%), bacterial (∼1%), viral (∼0.001%), ambiguous (∼0.31%), or unclassified (∼3%) based on SOAPaligner or blastn.
