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Graphical representation of real-time PCR data. Rn is the fluorescence of the reporter dye divided by the fluorescence of a passive reference dye; i.e., Rn is the reporter signal normalized to the fluorescence signal of ROX™. The figure demonstrate an amplification plot that show the variation of log (ΔRn) with PCR cycle number and as seen in the figure there are three positive cases that are above the threshold cycle (ct)
