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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Nutr Res. 2015 Apr 11;35(5):449–459. doi: 10.1016/j.nutres.2015.04.003

Table 1.

Diet composition of treatment diets

AIN-76A 1:1 5:1 10:1 20:1
Modified HFD HFD HFD HFD

Ingredient (g/kg)
Casein 200 165 165 165 165
DL Methionine 3 3 3 3 3
Lard 0 100 100 100 100
Coconut Oil 0 10.2 7.3 6.8 7.4
Corn Oil 15.6 .9 .5 22.5 50.1
Soybean Oil 3.6 1 41.1 28.1 3.8
Olive Oil 30.9 45 40 43.7 41.7
Flaxseed Oil - 38.5 4.9 1 -
Canola Oil - 7.4 9.2 1 -
Corn Starch 80 50 50 50 50
Maltodextrin 100 100 100 100 100
Sucrose 469.5 381.9 381.9 381.9 381.9
Cellulose 50 50 50 50 50
Vitamin Mix (AIN-76A) 10 10 10 10 10
Mineral Mix (AIN-76A) 35 35 35 35 35
Choline Bitartrate 2 2 2 2 2

Energy (kcal/g) 3.77 4.572 4.572 4.572 4.572

Energy (% kcal)
Carbohydrate 68.7 47 47 47 47
Fat 12.2 40 40 40 40
Protein 19.1 13 13 13 13

Fatty Acid Profile (g/kg)
Caprylic Acid (C8:0) 0 0.8 0.5 0.5 .6
Capric Acid (C10:0) 0 0.7 0.5 0.5 .5
Lauric Acid (C12:0) 0 4.8 3.5 3.2 3.5
Myristic Acid (C14:0) .004 3.1 2.5 2.4 2.6
Palmitic Acid (C16:0) 5.5 32.2 33.9 34.7 34.8
Palmitoleic Acid (C16:1) .4 3.3 3.2 3.3 3.3
Stearic Acid (C18:0) 1.05 16.2 16.7 16.3 15.6
Oleic Acid (C18:1) 27.1 85.9 86.1 86 85.9
Linoleic Acid (C18:2) 13.2 22.6 37.9 41.3 43.2
α-Linolenic Acid (C18:3) .66 22.6 7.6 4.13 2.2

% of Total Calories from SFAs 1.7% 12% 12% 12% 12%

% of Total Calories from MCFAs
(C6:0-C12:0)
- 1.3% .9% .9% 1%

% of Total Calories from LCSFAs
(C14:0-C20:0)
1.7% 10.7% 11.1% 11.1% 11%

% of Total Calories from USFAs 10.5% 28% 28% 28% 28%

% of Total Calories from MUFAs 7% 18.6% 18.6% 18.6% 18.6%

% of Total Calories from PUFAs 3.5% 9.4% 9.4% 9.4% 9.4%

% of Total Calories from n-3 FAs .16% 4.7% 1.6% .86% .46%

% of Total Calories from n-6 FAs 3.2 % 4.7% 7.8% 8.6% 9%

Cholesterol (mg/kg) 0 95 95 95 95

Ratio: MUFA:PUFA 2:1 2:1 2:1 2:1 2:1

Ratio: n-6:n-3 FA 20:1 1:1 5:1 10:1 20:1

SFAs, Saturated Fatty-Acids; MCSFAs, Medium-Chain Saturated Fatty Acids; LCSFAs, Long-Chain Saturated Fatty Acids; USFAs, Unsaturated Fatty Acids; MUFAs, Monounsaturated Fatty Acids; PUFAs, Polyunsaturated Fatty Acids.