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. 2015 Jun 1;10(6):e0126142. doi: 10.1371/journal.pone.0126142

Fig 4. (A) Real-time PCR quantification of C/EBPα gene expression in DM treatment of 3T3-L1 cells on days 0, 2, 4, 6, 8, and 10 with EEF2 used as endogenous control (∆Ct).

Fig 4

Data were normalized to C/EBPα gene expression of the day 0 group (∆∆Ct). (B): Image showing Western blot analysis (Odyssey Dual Infrared Imaging System (Li-Cor)) of C/EBPα on 0, 6, and 12 h, days 1, 2, 4, 6, 8 and 10. β-actin was used as an internal protein loading control. Quantification of C/EBPα was normalized to β-actin. Data are means ± SE (n = 3). Different letters represent treatment effects that were significantly different (P < 0.05).