Fig 5. Real-time PCR quantification of C/EBPα gene expression in 3T3-L1 cells on days 2 (A), 4 (B), 6 (C), 8 (D) and 10 (E).

Cells were treated with DM in the presence or absence of 0.01, 0.1, 1, 10, and 100 nM 1, 25 - (OH)₂D₃ and EEF2 was used as endogenous control (∆Ct). Data were normalized to C/EBPα gene expression of positive control (DM) at the corresponding time point (∆∆Ct). Data are means ± SE (n = 3). Different letters represent treatment effects that were significantly different (P < 0.05).