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. 2015 Jun 1;10(6):e0127072. doi: 10.1371/journal.pone.0127072

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© 2015 Heublein et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — The TR target gene CTNNB1 encoding β-Catenin was quantified in HCC3153 (BRCA1 ^mut) upon T3 stimulation. While T3 induced CTNNB1 in HCC3153 expressing THRA as well as THRB, CTNNB1 expression was significantly down-regulated upon T3 stimulation in HCC3153 silenced for THRA (A). Blocking TRs significantly reduced proliferation (B) and viability (C) of HCC3153 as quantified by BrdU (B) and WST-1 (C) assay, respectively. Reduced viability was also observed in HCC3153 silenced for THRA (D). Efficiency of THRA knockdown was validated on mRNA (E) and protein (F) level. THRB mRNA was not significantly altered in cells silenced for THRA (E). Mann-Whitney U Tests (B-D, F) and the REST2009 algorithm for determining relative gene expression (A, E) have been applied. Significant changes are indicated by stars (*) representative images are shown. scr—scrambled siRNA control, HiP—HighPerFect transfection reagent only control.