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. Author manuscript; available in PMC: 2015 Jun 2.

Published in final edited form as: Lung Cancer. 2014 Jun 16;85(2):282–292. doi: 10.1016/j.lungcan.2014.05.025

Fig. 3 — The effects of LATS2 silencing on ERK activation, Aktphosphorylation and p53 level. Cells were transfected with siRNA for LATS2 (siLATS2), Negative Control siRNA (siCont) or none (Mock Control) for 48 h. Cellular lysates were analyzed by Western blotting with the indicated antibodies. Number below each blot indicates fold change to mock control (n = 3). One asterisk indicates p < 0.05 determined by Student’s t-test. (A) Changes in phosphorylated ERK levels in cell lines after 48-h transfection with siRNAs described above. (B) Analysis of Thr308-phosphorylated Akt levels in cells after transfected with siRNAs described above. (C) Levels of Ser473-phosphorylated Akt in HKULC-2, H1648 and H1650 cells with or without LATS2 knockdown. D) The top left picture showed the basal levels of p53 in different cell lines. Modulation of p53 levels in these cells after transfected with siRNAs described above was demonstrated in the lower left picture and in the graph.

Fig. 3 — The effects of LATS2 silencing on ERK activation, Aktphosphorylation and p53 level. Cells were transfected with siRNA for LATS2 (siLATS2), Negative Control siRNA (siCont) or none (Mock Control) for 48 h. Cellular lysates were analyzed by Western blotting with the indicated antibodies. Number below each blot indicates fold change to mock control (n = 3). One asterisk indicates p < 0.05 determined by Student’s t-test. (A) Changes in phosphorylated ERK levels in cell lines after 48-h transfection with siRNAs described above. (B) Analysis of Thr308-phosphorylated Akt levels in cells after transfected with siRNAs described above. (C) Levels of Ser473-phosphorylated Akt in HKULC-2, H1648 and H1650 cells with or without LATS2 knockdown. D) The top left picture showed the basal levels of p53 in different cell lines. Modulation of p53 levels in these cells after transfected with siRNAs described above was demonstrated in the lower left picture and in the graph.