Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 1;212(6):953–970. doi: 10.1084/jem.20150002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Thompson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Loss of Dyrk1a adversely affects B cell development. (A) Mean bone marrow cellularity was assessed in Dyrk1a^f/f Mx1-Cre⁻ (Control) and Dyrk1a^f/f Mx1-Cre⁺ (CKO) mice 2 and 4 wk after pI:pC treatment; n = 6 mice per genotype, pooled from 3 independent cohorts of 2 mice per genotype. (B) Representative flow cytometry analysis of bone marrow for total pre–B (IgM⁻ gate, left) and large/small pre–B (pre–B gate, right) cells 4 wk after pI:pC treatment is shown. Numbers indicate percentages in each gate; mean percentages of each population among total bone marrow cells were quantified in (C); n = 6 mice per genotype, pooled from 3 independent cohorts of 2 mice per genotype. (D) Representative flow cytometry analysis of pre–B cell surface CD25 expression in bone marrow from Control and CKO mice 2 wk after pI:pC treatment is shown; n = 6 mice per genotype, pooled from 3 independent cohorts of 2 mice per genotype. (E) Mean number of colony forming units from total bone marrow 2 wk after pI:pC treatment as percent of Control for each colony type was calculated; n = 3 mice per genotype, with duplicate plates for each mouse. Data are representative of three independent experiments. (F) The mean numbers and phenotypes of colonies formed by WT bone marrow in the presence of indicated concentrations of EHT 1610 were calculated. Results depict duplicate plates for each condition, and represent 3 independent experiments. (G) Surface IL7R-α expression (H) and intracellular STAT5 phosphorylation (Y694, C) in small and large pre–B cells from the bone marrow of Control and CKO mice 2 wk after pI:pC treatment were assessed by flow cytometry. Data are representative of 3three mice per genotype. For all graphs, error bars depict SD. **, P < 0.01; ****, P < 0.0001.