Figure 3.

STAT3-deficient MAIT cells express normal levels of RORγt and PLZF. (A and B) PBMCs from normal controls or STAT3^MUT patients were stained with mAbs to identify MAIT cells or total T cells, and then expression of PLZF (A) and RORγt (B) was determined. Representative histogram plots of MAIT or T cells (CD3⁺Vα7.2-γδ⁻). Graphs show mean ± SEM (n = 6–10) of the MFI normalized to MFI of MAIT cells from controls in each experiment; **, P < 0.01. (C) RORC transcripts were determined by qPCR of sorted T cell populations (n = 5–9). Error bars indicate SEM.