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. 2015 Jun 1;212(6):855–864. doi: 10.1084/jem.20141992

Figure 6.

Figure 6.

IL-21 and IL-23 activate STAT3 and are required for the maintenance of iNKT and MAIT cells. (A) PBMCs from normal controls were stained for IL-6R, IL-12β1, and IL-21R on CD4+ T, iNKT, or MAIT cells (gray: isotype control, red: cytokine receptor). Graphs show fold increase in MFI over isotype control (mean ± SEM; n = 4–9). (B) Sorted MAIT (CD3+Vα7.2+CD161+) and non-MAIT (CD3+Vα7.2−CD161−) cells were stimulated with TAE beads, rested, and then stimulated with various cytokines for 20 min. After this time, cells were stained for phospho-STAT. Histograms show representative staining (gray: unstimulated, red: cytokine stimulated). Graphs show fold increase in MFI over unstimulated control (mean ± SEM; n = 3). (C and D) PBMCs from patients with IL12RB1, IL21R, or IFNGR1 mutations were stained for MAIT (CD3+Vα7.2+ CD161+; C) or iNKT (TCRVα24+ Vβ11+; D) cells. Data from normal controls and STAT3MUT patients from Fig. 1 are included for comparison. Each point represents a different individual; error bars indicate SEM; *, P < 0.05; ****, P < 0.0001.