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. Author manuscript; available in PMC: 2015 Jun 2.
Published in final edited form as: Biochemistry. 2013 Oct 1;52(41):7184–7195. doi: 10.1021/bi400560p

Figure 2.

Figure 2

Growth phenotypes of strains carrying the one-plasmid system. Photosynthetic (Ps) and respiration (Res) growth on enriched medium of R. capsulatus strains MT-RBC1 (RecA+) and BK-RBC1 (RecA−) harboring plasmid (A) pMTS1 carrying a wild-type petABC operon, (B) pBK6 carrying petAB1-B2C, and (C) pBK32 carrying the mutated petAB1F144R-B2H217LC fusion operons. In each case, the appropriate operon constructs are shown below the colony pictures, and mutations cyt b F144R and cyt b H217L are indicated with stars. Primers petA3 and petB4 (see the text) were used for PCR amplification.