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. Author manuscript; available in PMC: 2015 Jun 2.
Published in final edited form as: Biochemistry. 2013 Oct 1;52(41):7184–7195. doi: 10.1021/bi400560p

Figure 3.

Figure 3

Genetic rearrangements in a strain carrying the one-plasmid system. (A) PCR-amplified DNA mixtures using the petA3 and petB4 primers were run on a 1% agarose gel, and each lane corresponds to plasmid DNA extracted from independent colonies of strain pBK6/MT-RBC1 grown under Ps and Res conditions (Materials and Methods): lane 1, DNA size markers; lane 2, control plasmid pMTS1 carrying the petABC operon; lane 3, plasmid extracted from a colony carrying the one-plasmid system and growing as a wild type under photosynthetic (Ps) conditions; lane 4, plasmid extracted from a colony carrying the one-plasmid system and growing poorly under Ps conditions; lanes 5–11, plasmids extracted from similar colonies growing under respiratory (Res) conditions. The 1.5 and 3 kb bands correspond to wild-type petB and the petB1-petB2 fusion, respectively. (B) SDS-PAGE and immunoblot analyses of chromatophore membranes (40 μg of total proteins) from cells harboring the one-plasmid system: lane 1, pMTS1/MT-RBC1; lane 2, MT-RBC1; lanes 3–8, Ps- and Res-grown colonies of pBK6/MT-RBC1 compared for their cytochrome b contents using anti-cytochrome b (anti b) and anti-Strep (anti Strep) antibodies.