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. 2015 Jun 1;10(6):e0128704. doi: 10.1371/journal.pone.0128704

Fig 3. Effect of the buffer on stathmin depolymerizing activity.

Fig 3

Samples containing various concentrations of stahtmin (0–15μM) added to paclitaxel stabilized microtubules (10μM tubulin) were sedimented and the amount of tubulin in the supernatants (SN) and pellets (P) was analyzed by SDS PAGE. The two representative gels displayed above the graph correspond to samples in the presence of 0, 5, 10 or 15μM stathmin, in PEM buffer (upper gel) or PM buffer (lower gel). Quantification of sedimentable tubulin polymers assembled in PM buffer (black squares) vs. PEM buffer (black circle) corresponds to averages of 0, 1, 2, 3, 5, 7.5, 10 and 15 μM stathmin in PM buffer and 0, 2, 5, 7.5, 10 and 15 μM stathmin in PEM buffer.