Fig 1. Levels of alcohol metabolic enzymes in the liver of tree shrews analyzed by western blot after treatment with water or alcohol solutions for 14 days.

Feeding alcohol can cause significant changes in the level of various alcohol metabolic enzymes. (A) The protein bands of the alcohol metabolic enzymes detected by western blot; (B–F) Protein expressions of ADH1, ALDH2, CYP2E1, UGT1A1 and Nrf2 relative to the internal reference protein β-actin with no, low, and high levels of alcohol treatment. All data are expressed as the mean ± SD, an asterisk (*) denotes a significant difference between one another with p < 0.05.