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. 2015 Jun 1;10(6):e0128227. doi: 10.1371/journal.pone.0128227

Fig 3. Sequence of cDNA generated by minigene.

Fig 3

(A) Schematic diagram of the FLAG-tagged MSX1 gene. Thick cylinders, exons; blue cylinder, FLAG-tag; asterisk, position at nucleotide substitution; red cylinder, 7-nucleotide insertion. (B) Electropherograms of the MSX1 cDNA isolated from the COS7 cells transfected with MSX1 minigene plasmids; wild-type (II-1) and c.452-9G>A (II-2). (C) Predicted amino acid sequences of wild-type (upper) and p.R151fsX20 (lower). The 7-bp insertion and 19 additional amino acids residues in the C-terminus are highlighted in red.