Table IV.

Analysis of fecal bile acids in individual wild-type and Cyp7^−/− adult mice

Retention timea	Bile acidb	Cyp7 genotype
		+/+	+/+	+/+	+/+	+/+	−/−	−/−	−/−	−/−	−/−
MU		μg/gfeces
31.18	3α-Hydroxy-5β-cholanoic acid (lithocholic acid)	12	23	26	22	31	8	6	6	ND	4
31.61	Unknown bile acid	NDc	12	23	18	18	ND	ND	4	ND	ND
31.76	3,12-Dihydroxy bile acid	21	27	26	34	32	3	6	5	ND	4
31.88	3α-12α-Dihydroxy-5β-cholanoic acid (deoxycholic acid)	242	276	319	411	333	41	73	78	10	35
32.00	3α,7α,12α-Trihydroxy-5α-cholanoic acid (allo-cholic acid)	18	11	11	ND	9	ND	ND	6	8	8
32.15	3α,7α-Dihydroxy-5β-cholanoic acid (chenodeoxycholic acid)	ND	8	11	ND	10	8	6	6	7	4
32.20	3α,6β,7α-Trihydroxy-5β-cholanoic acid (α-muricholic acid)	45	36	56	57	69	ND	ND	ND	ND	ND
32.26	3α,7α,12α-Trihydroxy-5β-cholanoic acid (cholic acid)	127	137	158	181	132	43	62	70	188	117
32.29	3α,6α-Dihydroxy-5β-cholanoic acid (hyodeoxycholic acid)	60	106	129	112	114	69	64	71	59	51
32.45	Unknown bile acid	13	20	24	20	20	10	7	8	7	8
32.51	3α,7β-Dihydroxy-5β-cholanoic acid (ursodeoxycholic acid)	ND	ND	11	ND	ND	6	ND	14	ND	ND
32.60	3β,12α-Dihydroxy-5α-cholanoic acid	12	16	17	21	20	ND	ND	ND	ND	ND
32.84	3α,7α,12α-Trihydroxy-5β-homocholanoic acid (homocholic acid)	8	ND	14	15	10	ND	4	ND	ND	ND
33.06	12-Oxo-3α-hydroxy-5β-cholanoic acid	65	44	85	75	61	26	35	30	23	26
33.22	3α,6β,7β-Trihydroxy-5β-cholanoic acid (β-muricholic acid)	76	66	77	141	81	45	54	53	71	75
33.79	7-Oxo-3α,12α-dihydroxy-5β-cholanoic acid	11	ND	9	16	6	ND	5	6	6	5
34.30	3α,6α,7β-Trihydroxy-5β-cholanoic acid (ω-muricholic acid)	117	140	157	194	136	39	34	29	29	35
34.55	3α,6α,7β-Trihydroxy-5α-cholanoic acid (allo-ω-muricholic acid)	27	33	41	43	34	6	7	4	6	6
	Total bile acids	854	955	1194	1360	1116	304	363	390	414	378

^aBile acids are listed based on their retention times as methyl ester-trimethylsilyl ethers relative to a homologous series of n-alkanes, referred to as the methylene unit (MU) value.

^bChemical structures were determined by electron ionization-gas chromatography-mass spectrometry.

^cND, not detected.