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. Author manuscript; available in PMC: 2016 Jul 15.
Published in final edited form as: J Neuroimmunol. 2015 May 13;284:57–66. doi: 10.1016/j.jneuroim.2015.05.008

Figure 5. IL-10 pre-treatment attenuated TNF-α-induced loss of claudin-5 expression.

Figure 5

Analysis of immunofluorescence studies showed that (A, F) control bEnd.3 cells express claudin-5 at the cell margins (arrows). (B) TNF-α (1.0 ng/ml) exposure for 24 h induced loss of paracellular claudin-5 expression (asterisk). (C, D) 30 min pre-treatment with IL-10 (1.0 & 10 ng/ml) prevented the loss of paracellular claudin-5 expression (arrows) induced by TNF-α. (E) IL-10 (1.0 ng/ml) alone had no effect on claudin-5 expression (arrow). (G) TNF-α (10 ng/ml) induced loss of paracellular claudin-5 expression. (H, I) Pre-treatment with IL-10 (1.0–10 ng/ml) prevented TNF-α-induced loss of paracellular claudin-5 (arrows). (J) IL-10 (10 ng/ml) alone had no effect on claudin-5 expression (arrow). Scale bar = 25 μm (refers to A–J).