Skip to main content
. 2015 Jun 1;10(6):e0128558. doi: 10.1371/journal.pone.0128558

Fig 1. Establishment of stable cell lines expressing CSB-TAP protein and Identification of proteins that co-purified with CSB-TAP.

Fig 1

(A) Western blot showing the expression of endogenous CSB full-length (CSB fl) and CSB-PGBD3 proteins in wild type MRC5 fibroblasts and either chimeric CSB-TAP protein (CSIAN TAP/CSB) or TAP domain (CSIAN TAP) in CSB-deficient fibroblasts CSIAN after stable transfection. TAP tagged proteins were detected using rabbit polyclonal anti TAP tag (CAB 1001, Pierce) and endogenous CSB, either fl and PGBD3 isoforms, using rabbit polyclonal anti-ERCC6 (H300, Santacruz). (B) UV survival demonstrated the complementation of UV survival in CSB-TAP transfected cells. Clonogenic survival after UV exposure in MRC5, CSIAN and isogenic clonal populations of CSIAN transfectant cell lines is shown as the percentage of survival. Results shown are the average values of three independent experiments. (C) Silver staining of proteins associated with CSB-TAP and TAP that were isolated by tandem affinity purification and, separated on a 4–12% Bis-Tris gel.