Fig 4. Co-immunoprecipitations studies.

Co-immunoprecipitation was performed using the lysates of CSIAN cells, transiently expressing flag tagged CSB and myc-tagged proteins of interest. Whole cell extracts, prepared 24 hr post transfection, were immunoprecipitated for either CSB or putative interacting proteins using respectively Flag or c-Myc monoclonal antibody conjugated agarose resins, followed by Western blot analysis using anti-myc and anti-flag antibodies respectively. * two isoforms p72 and p82 arise from ddx17 gene through the use of different in-frame translation initiation codons. (WCE, whole cellular extract; FT, Flow-Through; IP, immunoprecipitation).